G2 phase
G2 phase, or Gap 2 phase, is the second subphase of Interphase in the cell cycle directly preceding mitosis. It follows the successful completion of S phase, during which the cell’s DNA is replicated. G2 phase ends with the onset of prophase, the first phase of mitosis in which the cell’s chromatin condenses into chromosomes.
Contents
1 Overview
2 G2/M checkpoint
3 End of G2/entry into mitosis
4 References
Overview
G2 phase is a period of rapid cell growth and protein synthesis during which the cell prepares itself for mitosis. Curiously, G2 phase is not a necessary part of the cell cycle, as some cell types (particularly young Xenopus embryos [1] and some cancers [2]) proceed directly from DNA replication to mitosis. Though much is known about the genetic network which regulates G2 phase and subsequent entry into mitosis, there is still much to be discovered concerning its significance and regulation, particularly in regards to cancer. One hypothesis is that the growth in G2 phase is regulated as a method of cell size control. Fission yeast (Schizosaccharomyces pombe) has been previously shown to employ such a mechanism, via Cdr2-mediated spatial regulation of Wee1 activity.[3] Though Wee1 is a fairly conserved negative regulator of mitotic entry, no general mechanism of cell size control in G2 has yet been elucidated.
Biochemically, the end of G2 phase occurs when a threshold level of active cyclin B1/CDK1 complex, also known as Maturation promoting factor (MPF) has been reached.[4] The activity of this complex is tightly regulated during G2. In particular, the G2 checkpoint arrests cells in G2 in response to DNA damage through inhibitory regulation of CDK1.
G2/M checkpoint
In vertebrate cells, the G2/M DNA damage checkpoint consists of an arrest of the cell in G2 just before mitotic entry in response to genotoxic stress (such as UV radiation, oxidative stress, DNA intercalating agents, etc.) in both a p53-dependent and p53-independent manner.[5] DNA damage signals cause activation of the transcription factor p53. CDK1 is directly inhibited by three transcriptional targets of p53: p21, Gadd45, and 14-3-3σ. Inactive Cyclin B1/CDK1 is sequestered in the nucleus by p21,[6] while active Cyclin B1/CDK1 complexes are sequestered in the cytoplasm by 14-3-3σ.[5] Gadd45 disrupts the binding of Cyclin B1 and CDK1 through direct interaction with CDK1. P53 also transcriptionally represses CDK1.[5]
P53-independent G2 arrest is mainly affected through the actions of Chk1 kinase. DNA damage is sensed by ATM and ATR (Rad3 and Mec1 in yeast), which then signal to Chk1 and Chk2. Chk1 then mediates the degradation of cdc25A, an activator of CDK1.[7] ATR/ATM also activate p53, indicating that these pathways may act synergistically in regulating G2 arrest.[5]
Both p53-dependent and p53-independent cell cycle arrest are not specific to G2; these same proteins function upstream in DNA damage checkpoints in G1 and S phase as well. In yeast, which has no p53 homolog, G2 arrest functions through the p53-independent pathway.[5]
End of G2/entry into mitosis
Mitotic entry is determined by a threshold level of active cyclin B1/CDK1 complex. In vertebrates, there are five cyclin B isoforms (B1, B2, B3, B4, and B5), but specific role of each of these isoforms in regulating mitotic entry is still unclear. It is known that cyclin B1 can compensate for loss of both cyclin B2 (and vice versa in Drosophila). Cyclin B1/CDK1 activity is regulated both spatially and temporally during G2 phase to ensure proper entry into mitosis.[8]
Cyclin B1 transcription begins at the end of S phase after DNA replication. Its promoter contains consensus binding sequences for a number of transcription factors, including p53, p21, Ets, Ap-1, NF-Y, c-Myc, TFE3, and USF.[8] Cyclin B1 accumulates in the cytoplasm throughout G2, where it binds to and activates CDK1's kinase activity. CDK1 activity is modulated primarily through regulation of its inhibitory phosphorylation sites at Thr14 and Tyr15. Wee1 and Myt1 phosphorylate these two residues, with Wee1 acting on the Tyr15 site and Myt1 acting predominantly on the Thr14 site. However, Myt1 has a separate inhibitory effect on CDK1; it can also sequester CDK1 in the cytoplasm via interaction with Myt1's C-terminal domain.[9] CDK1 is dephosphorylated primarily through the actions of Cdc25, which can dephosphorylate both the Thr14 and Tyr15 residues of CDK1.[9] There are three isoforms of Cdc25 (A, B, and C) in mammalian cells, all of which have been shown to have roles in regulation of G2 phase.[8][9]
CDK1, in turn, phosphorylates and modulates the activity of Wee1 and the Cdc25 isoforms A and C. Specifically, CDK1 phosphorylation inhibits Wee1 kinase activity,[8] activates Cdc25C phosphatase activity, and stabilizes Cdc25A.[10] Thus, CDK1 forms a positive feedback loop with Cdc25 and a double negative feedback loop with Wee1 (essentially a net positive feedback loop). These loops encode a hysteretic bistable switch in CDK1 activity relative to cyclin B1 levels. It is thought that this hysteretic behavior ensures that cells commit to mitosis even if cyclin B1 levels falter.[4]
In mammals, cyclin B1/CDK1 translocation to the nucleus is activated by phosphorylation of five serine sites on cyclin B1's cytoplasmic retention site (CRS): S116, S26, S128, S133, and S147. In Xenopus laevis, cyclin B1 contains four analogous CRS serine phosphorylation sites (S94, S96, S101, and S113) indicating that this mechanism is highly conserved. Nuclear export is also inactivated by phosphorylation of cyclin B1's nuclear export signal (NES).[8] The regulators of these phosphorylation sites are still largely unknown but several factors have been identified, including extracellular signal-regulated kinases (ERKs), PLK1, and CDK1 itself.[8][11] Upon reaching some threshold level of phosphorylation, translocation of cyclin B1/CDK1 to the nucleus is extremely rapid.[12] Once in the nucleus, cyclin B1/CDK1 phosphorylates many targets in preparation for mitosis, including histone H1, nuclear lamins, centrosomal proteins, and microtubule associated proteins (MAPs).[8]
Recently, evidence has emerged suggesting a more important role for cyclin A2/CDK complexes in regulating entry into mitosis. Cyclin A2/CDK2 activity begins in early S phase and increases during G2.[13] Cdc25B has been shown to dephosphorylate Tyr15 on CDK2 in early-to-mid G2 in a manner similar to the aforementioned CDK1 mechanism.[14] Downregulation of cyclin A2 in U2OS cells increases Wee1 activity and lowers Plk1 and Cdc25C activity.[15] However, cyclin A2/CDK complexes do not function strictly as activators of cyclin B1/CDK1 in G2, as CDK2 has been shown to be required for activation of the p53-independent G2 checkpoint activity, perhaps through a stabilizing phosphorylation on Cdc6.[16] CDK2-/- cells also have aberrantly high levels of Cdc25A.[16] Cyclin A2/CDK1 has also been shown to mediate proteosomal destruction of Cdc25B.[17] These pathways are often deregulated in cancer.[15]
References
^ Alberts, B et al. Molecular Biology of the Cell. New York: Garland Science, 3rd Ed (1994).
^ Liskay, R. M. (1977). "Absence of a measurable G2 phase in two Chinese hamster cell lines". Proceedings of the National Academy of Sciences of the United States of America. 74 (4): 1622–1625. Bibcode:1977PNAS...74.1622L. doi:10.1073/pnas.74.4.1622. PMC 430843. PMID 266201..mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{quotes:"""""""'""'"}.mw-parser-output .citation .cs1-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-subscription a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration{color:#555}.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration span{border-bottom:1px dotted;cursor:help}.mw-parser-output .cs1-ws-icon a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Wikisource-logo.svg/12px-Wikisource-logo.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output code.cs1-code{color:inherit;background:inherit;border:inherit;padding:inherit}.mw-parser-output .cs1-hidden-error{display:none;font-size:100%}.mw-parser-output .cs1-visible-error{font-size:100%}.mw-parser-output .cs1-maint{display:none;color:#33aa33;margin-left:0.3em}.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-format{font-size:95%}.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-left{padding-left:0.2em}.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-right{padding-right:0.2em})
^ Moseley, J. B.; Mayeux, A.; Paoletti, A.; Nurse, P. (2009). "A spatial gradient coordinates cell size and mitotic entry in fission yeast". Nature. 459 (7248): 857–860. Bibcode:2009Natur.459..857M. doi:10.1038/nature08074. PMID 19474789.
^ ab Sha, W.; Moore, J.; Chen, K.; Lassaletta, A. D.; Yi, C. S.; Tyson, J. J.; Sible, J. C. (2002). "Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy of Sciences. 100 (3): 975–980. Bibcode:2002PNAS..100..975S. doi:10.1073/pnas.0235349100. PMC 298711. PMID 12509509.
^ abcde Taylor, W. R.; Stark, G. R. (2001). "Regulation of the G2/M transition by p53". Oncogene. 20 (15): 1803–1815. doi:10.1038/sj.onc.1204252. PMID 11313928.
^ Charrier-Savournin, F. B.; Château, M.; Gire, V.; Sedivy, J.; Piette, J.; Dulić, V. (2004). "p21-Mediated Nuclear Retention of Cyclin B1-Cdk1 in Response to Genotoxic Stress". Molecular Biology of the Cell. 15 (9): 3965–3976. doi:10.1091/mbc.E03-12-0871. PMC 515331. PMID 15181148.
^ Xiao, Z.; Chen, Z.; Gunasekera, A.; Sowin, T.; Rosenberg, S.; Fesik, S.; Zhang, H. (2003). "Chk1 Mediates S and G2 Arrests through Cdc25A Degradation in Response to DNA-damaging Agents". Journal of Biological Chemistry. 278 (24): 21767–21773. doi:10.1074/jbc.M300229200. PMID 12676925.
^ abcdefg Porter, L. A.; Donoghue, D. J. (2003). "Cyclin B1 and CDK1: nuclear localization and upstream regulators". Progress in Cell Cycle Research. 5: 335–347. PMID 14593728.
^ abc Chow, J. P. H.; Siu, W.; Ho, H.; Ma, K.; Ho, C.; Poon, R. (2003). "Differential Contribution of Inhibitory Phosphorylation of CDC2 and CDK2 for Unperturbed Cell Cycle Control and DNA Integrity Checkpoints". Journal of Biological Chemistry. 278 (42): 40815–40828. doi:10.1074/jbc.M306683200. PMID 12912980.
^ Mailand, N.; Podtelejnikov, A. V.; Groth, A.; Mann, M.; Bartek, J.; Lukas, J. (2002). "Regulation of G2/M events by Cdc25A through phosphorylation-dependent modulation of its stability". The EMBO Journal. 21 (21): 5911–5920. doi:10.1093/emboj/cdf567. PMC 131064. PMID 12411508.
^ Chambard, J.; Lefloch, R.; Pouyssegur, J.; Lenormand, P. (2007). "ERK implication in cell cycle regulation". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1773 (8): 1299–1310. doi:10.1016/j.bbamcr.2006.11.010. PMID 17188374.
^ Lindqvist, A. (2010). "Cyclin B–Cdk1 activates its own pump to get into the nucleus". The Journal of Cell Biology. 189 (2): 197–199. doi:10.1083/jcb.201003032. PMC 2856906. PMID 20404105.
^ Hu, B.; Mitra, J.; Van Den Heuvel, S.; Enders, G. H. (2001). "S and G2 Phase Roles for Cdk2 Revealed by Inducible Expression of a Dominant-Negative Mutant in Human Cells". Molecular and Cellular Biology. 21 (8): 2755–2766. doi:10.1128/MCB.21.8.2755-2766.2001. PMC 86906. PMID 11283255.
^ Goldstone, S.; Pavey, S.; Forrest, A.; Sinnamon, J.; Gabrielli, B. (2001). "Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR". Oncogene. 20 (8): 921–932. doi:10.1038/sj.onc.1204177. PMID 11314027.
^ ab Mitra, J.; Enders, G. H. (2004). "Cyclin A/Cdk2 complexes regulate activation of Cdk1 and Cdc25 phosphatases in human cells". Oncogene. 23 (19): 3361–3367. doi:10.1038/sj.onc.1207446. PMC 1924680. PMID 14767478.
^ ab Chung, J. H.; Bunz, F.; Biggins, S. (2010). "Cdk2 is Required for p53-Independent G2/M Checkpoint Control". PLoS Genetics. 6 (2): e1000863. doi:10.1371/journal.pgen.1000863. PMC 2829054. PMID 20195506.
^ Baldin, V.; Cans, C.; Knibiehler, M.; Ducommun, B. (1997). "Phosphorylation of human CDC25B phosphatase by CDK1-cyclin a triggers its proteasome-dependent degradation". The Journal of Biological Chemistry. 272 (52): 32731–32734. doi:10.1074/jbc.272.52.32731. PMID 9407044.
